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biotinylated goat anti human dkk1  (R&D Systems)


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    Structured Review

    R&D Systems biotinylated goat anti human dkk1
    Biotinylated Goat Anti Human Dkk1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/goat+anti+human+dkk1+antibody/Human+Dkk-1+Biotinylated+Antibody/us11952418-420-13-17
    Average 93 stars, based on 18 article reviews
    biotinylated goat anti human dkk1 - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Membrane:

    Article Title: Dickkopf-1 (DKK1) is a widely expressed and potent tumor-associated antigen in multiple myeloma
    Article Snippet: .. After transfer to nitrocellulose membrane and subsequent blocking, the membranes were immunoblotted with goat anti-human DKK1 antibody (R&D Systems) and visualized with horseradish peroxidase (HRP)–conjugated donkey anti–goat IgG antibody (Santa Cruz Biotechnology, Santa Cruz, CA), followed by enhanced chemiluminescence (Pierce Biotechnology, Rockford, IL) and autoradiography. ..

    Blocking Assay:

    Article Title: Dickkopf-1 (DKK1) is a widely expressed and potent tumor-associated antigen in multiple myeloma
    Article Snippet: .. After transfer to nitrocellulose membrane and subsequent blocking, the membranes were immunoblotted with goat anti-human DKK1 antibody (R&D Systems) and visualized with horseradish peroxidase (HRP)–conjugated donkey anti–goat IgG antibody (Santa Cruz Biotechnology, Santa Cruz, CA), followed by enhanced chemiluminescence (Pierce Biotechnology, Rockford, IL) and autoradiography. ..

    Article Title: Designation of a Novel DKK1 Multiepitope DNA Vaccine and Inhibition of Bone Loss in Collagen-Induced Arthritic Mice
    Article Snippet: .. After blocking with 5% horse serum, the sections were incubated with a goat anti-human DKK1 antibody (5 μ g/mL, R&D Systems) overnight at 4°C. .. The next day, the sections were incubated with a HRP-conjugated donkey anti-goat IgG (1 : 1000, ProteinTech Group) for 1 h at room temperature.

    Autoradiography:

    Article Title: Dickkopf-1 (DKK1) is a widely expressed and potent tumor-associated antigen in multiple myeloma
    Article Snippet: .. After transfer to nitrocellulose membrane and subsequent blocking, the membranes were immunoblotted with goat anti-human DKK1 antibody (R&D Systems) and visualized with horseradish peroxidase (HRP)–conjugated donkey anti–goat IgG antibody (Santa Cruz Biotechnology, Santa Cruz, CA), followed by enhanced chemiluminescence (Pierce Biotechnology, Rockford, IL) and autoradiography. ..

    Concentration Assay:

    Article Title: Compositions and methods of use for antibodies of dickkopf-1
    Article Snippet: .. The anti-DKK1 HuCAL® Fabs (final concentration 20 μg/ml) and the goat anti-human DKK1 antibody (R&D Systems) used as a positive control (final concentration 1.5 μg/ml) are diluted in CM. ..

    Positive Control:

    Article Title: Compositions and methods of use for antibodies of dickkopf-1
    Article Snippet: .. The anti-DKK1 HuCAL® Fabs (final concentration 20 μg/ml) and the goat anti-human DKK1 antibody (R&D Systems) used as a positive control (final concentration 1.5 μg/ml) are diluted in CM. ..

    Incubation:

    Article Title: Designation of a Novel DKK1 Multiepitope DNA Vaccine and Inhibition of Bone Loss in Collagen-Induced Arthritic Mice
    Article Snippet: .. After blocking with 5% horse serum, the sections were incubated with a goat anti-human DKK1 antibody (5 μ g/mL, R&D Systems) overnight at 4°C. .. The next day, the sections were incubated with a HRP-conjugated donkey anti-goat IgG (1 : 1000, ProteinTech Group) for 1 h at room temperature.

    Electrochemiluminescence:

    Article Title: Low-density lipoprotein receptor-related protein 5 (LRP5) mediates the prostate cancer-induced formation of new bone.
    Article Snippet: .. DKK1 was detected by electrochemiluminescence (Amersham, Arlington-Heights, IL, USA) with goat anti-human DKK1 antibody (R&D Systems). .. In vivo prostate cancer intrabone model MDA PCa 2b and PC-3 cells were injected into the femurs of male SCID mice (Charles River Laboratories, Wilmington, MA, USA) as previously described (Yang et al., 2001).



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    R&D Systems goat anti human dkk1 neutralizing antibody
    PTC-209 inhibits osteogenesis via upregulation of <t>DKK1.</t> a PTC-209 significantly inhibited osteoblast formation in a dose-dependent manner verified by reduced alkaline phosphatase activity and matrix mineralization at days 14 and 21 of differentiation, respectively. Images are representative for three independent experiments. b Treatment with PTC-209 increased DKK1 expression in developing osteoblasts at day 14 of osteogenesis. c The inhibitory effect of PTC-209 on osteoblast activity was partially overcome by concurrent anti-DKK1 antibody treatment. *** P < 0.001, ** P < 0.01 and * P < 0.05 vs DMSO control; # P < 0.05
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    R&D Systems biotinylated goat anti human dkk1 igg
    PTC-209 inhibits osteogenesis via upregulation of <t>DKK1.</t> a PTC-209 significantly inhibited osteoblast formation in a dose-dependent manner verified by reduced alkaline phosphatase activity and matrix mineralization at days 14 and 21 of differentiation, respectively. Images are representative for three independent experiments. b Treatment with PTC-209 increased DKK1 expression in developing osteoblasts at day 14 of osteogenesis. c The inhibitory effect of PTC-209 on osteoblast activity was partially overcome by concurrent anti-DKK1 antibody treatment. *** P < 0.001, ** P < 0.01 and * P < 0.05 vs DMSO control; # P < 0.05
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    Image Search Results


    PTC-209 inhibits osteogenesis via upregulation of DKK1. a PTC-209 significantly inhibited osteoblast formation in a dose-dependent manner verified by reduced alkaline phosphatase activity and matrix mineralization at days 14 and 21 of differentiation, respectively. Images are representative for three independent experiments. b Treatment with PTC-209 increased DKK1 expression in developing osteoblasts at day 14 of osteogenesis. c The inhibitory effect of PTC-209 on osteoblast activity was partially overcome by concurrent anti-DKK1 antibody treatment. *** P < 0.001, ** P < 0.01 and * P < 0.05 vs DMSO control; # P < 0.05

    Journal: Journal of Hematology & Oncology

    Article Title: Targeting of BMI-1 with PTC-209 shows potent anti-myeloma activity and impairs the tumour microenvironment

    doi: 10.1186/s13045-016-0247-4

    Figure Lengend Snippet: PTC-209 inhibits osteogenesis via upregulation of DKK1. a PTC-209 significantly inhibited osteoblast formation in a dose-dependent manner verified by reduced alkaline phosphatase activity and matrix mineralization at days 14 and 21 of differentiation, respectively. Images are representative for three independent experiments. b Treatment with PTC-209 increased DKK1 expression in developing osteoblasts at day 14 of osteogenesis. c The inhibitory effect of PTC-209 on osteoblast activity was partially overcome by concurrent anti-DKK1 antibody treatment. *** P < 0.001, ** P < 0.01 and * P < 0.05 vs DMSO control; # P < 0.05

    Article Snippet: Goat anti-human DKK1 neutralizing antibody and normal goat IgG were purchased from R&D Systems, dissolved in PBS and stored at −20 °C.

    Techniques: Activity Assay, Expressing, Control